کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1932592 | 1536788 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regulation of interleukin-4 signaling by extracellular reduction of intramolecular disulfides
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
LPSDTTIL-4N-ethylmaleimideGSHNACPDIIL-4RT-helperN-acetylcysteine - N-استیل سیستئینTrx1 - TRX1interleukin-4 - اینترلوکین -4thioredoxin reductase - تریودوکسین ردوکتازThioredoxin 1 - تیورودکسین 1Disulfides - دیسولفیدdithiothreitol - دیتیوتریتولCytokines - سیتوکین هاlipopolysaccharide - لیپوپلی ساکاریدRedox regulation - مقررات RedoxNEM - نهprotein disulfide isomerase - پروتئین دیسولفید ایزومرازreduced glutathione - کاهش گلوتاتیونInterleukin-4 receptor - گیرنده اینترلوکین -4
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Interleukin-4 (IL-4) contains three structurally important intramolecular disulfides that are required for the bioactivity of the cytokine. We show that the cell surface of HeLa cells and endotoxin-activated monocytes can reduce IL-4 intramolecular disulfides in the extracellular space and inhibit binding of IL-4 to the IL-4Rα receptor. IL-4 disulfides were in vitro reduced by thioredoxin 1 (Trx1) and protein disulfide isomerase (PDI). Reduction of IL-4 disulfides by the cell surface of HeLa cells was inhibited by auranofin, an inhibitor of thioredoxin reductase that is an electron donor to both Trx1 and PDI. Both Trx1 and PDI have been shown to be located at the cell surface and our data suggests that these enzymes are involved in catalyzing reduction of IL-4 disulfides. The pro-drug N-acetylcysteine (NAC) that promotes T-helper type 1 responses was also shown to mediate the reduction of IL-4 disulfides. Our data provides evidence for a novel redox dependent pathway for regulation of cytokine activity by extracellular reduction of intramolecular disulfides at the cell surface by members of the thioredoxin enzyme family.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 390, Issue 4, 25 December 2009, Pages 1272-1277
Journal: Biochemical and Biophysical Research Communications - Volume 390, Issue 4, 25 December 2009, Pages 1272-1277
نویسندگان
Sophie Curbo, Raphaël Gaudin, Mattias Carlsten, Karl-Johan Malmberg, Marita Troye-Blomberg, Niklas Ahlborg, Anna Karlsson, Magnus Johansson, Mathias Lundberg,