کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1932945 1050598 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structure of the Enterococcus faecalis EIIAgnt PTS component
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Structure of the Enterococcus faecalis EIIAgnt PTS component
چکیده انگلیسی

In Eubacteria, the utilization of a number of extracellular carbohydrates is mediated by sugar specific phosphoenolepyruvate (PEP) dependent sugar phosphotransferase systems (PTSs), which simultaneously import und phosphorylate their target sugars. Here, we report the crystal structure of the EIIAgnt component of the so far little investigated Enterococcus faecalis gluconate specific PTS. The crystal structure shows a tightly interacting dimer of EIIAgnt which is structurally similar to the related EIIAman from Escherichia coli. Homology modeling of E. faecalis HPr, EIIBman and their complexes with EIIAman suggests that despite moderate sequence identity between EIIAman and EIIAgnt, the active sites closely match the situation observed in the E. coli system with His-9 of EIIAgnt being the likely phosphoryl group carrier. We therefore propose that the phosphoryl transfer reactions involving EIIAgnt proceed according to a mechanism analog to the one described for E. coli EIIAman.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 388, Issue 4, 30 October 2009, Pages 626–629
نویسندگان
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