Increased ribosomal biogenesis induces pancreatic β cell failure in mice model of type 2 diabetes

AimTo study the changes in gene expression by pancreatic β cells under insulin resistance conditions.MethodAn exhaustive gene expression analysis was performed, using isolated pancreatic islets of obese diabetic model Lepr−/− mice. Overexpression of cyclin D2 was induced in cells from the pancreatic β cell line, namely, INS-1.ResultsThrough a gene expression analysis using islets isolated from db/db mice, we found a significant increase in the expression of ribosome-related molecules. In addition, increased expression of cyclin D2 was found at certain protein levels. As INS-1 cells were induced to overexpress cyclin D2, we found an increase in the expression of ribosome-related molecules. Concurrently, an increase in the expression of endoplasmic reticulum stress (ER stress)-related molecules was also found.ConclusionIn cases of pancreatic β cell hyperplasia associated with insulin resistance, ribosomal biogenesis is increased, and ER stress is induced.