کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1933569 1050617 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Pro-apoptotic activity and mono-/diubiquitylation of Xenopus Bid in egg extracts
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Pro-apoptotic activity and mono-/diubiquitylation of Xenopus Bid in egg extracts
چکیده انگلیسی

Apoptosis in Xenopus egg extracts is carried out by maternally stockpiled materials, but the contributions of endogenous apoptosis regulators are still poorly characterized. Here we examined the physiological role of Xenopus Bid (xBid), a pro-apoptotic BH3-only member of Bcl-2 family proteins. We found that endogenous xBid was a physiological accelerator of apoptosis in egg extracts. Interestingly, xBid was mono-/diubiquitylated but not degraded by proteasome in egg extracts, and we identified three ubiquitylated Lys residues in the N-terminal propeptide region. Comparison with human Bid suggested that mono-/diubiquitylation is a specific feature of xBid.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 384, Issue 4, 10 July 2009, Pages 491–494
نویسندگان
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