کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1934355 1050638 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
How calcium controls microtubule anisotropic phase formation in the presence of microtubule-associated proteins in vitro
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
How calcium controls microtubule anisotropic phase formation in the presence of microtubule-associated proteins in vitro
چکیده انگلیسی

Here we show a new effect of Ca2+ on microtubule morphology: Ca2+ can cause smooth curving of microtubules in the presence of microtubule-associated proteins (MAPs). In vitro, microtubules self-organize, forming complex dissipative structures. Such structures may be strongly affected by relatively weak external factors. A factor such as Ca2+ potentially influences spatiotemporal patterns of microtubule assembly, but the dynamics are unclear. We tested Ca2+ effects on microtuble formation. Using EM, microtubule length, curvature, and alignment and were measured in two systems: 2 mg/ml microtubule protein containing MAPs and 1 mM EGTA with and without 1 mM Ca2+. The two systems were then tested using light scattering. In low Ca2+, a birefringent microtubular pattern is seen, increasing with polymerization. When 1 mM Ca2+ is added to the solution. anisotropic phase is prevented without microtubule disruption. This demonstrates an additional mechanism by which Ca2+ can alter the dynamics and morphology of microtules.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 381, Issue 2, 3 April 2009, Pages 224–228
نویسندگان
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