کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1934406 1050639 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection and chromatographic removal of lipopolysaccharide in preparations of multifunctional galectins
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Detection and chromatographic removal of lipopolysaccharide in preparations of multifunctional galectins
چکیده انگلیسی

The functional spectrum of human galectins is currently explored, with a wide range of activities being described. The role of galectin-3 as adhesin for bacteria is based on its strong binding to lipopolysaccharides (LPSs), which brings the possibility of such a contamination in galectin preparations to awareness. This assumption was verified in three independent functional assay systems using polymyxin B as inhibitor of LPS-dependent effects. Moreover, a commercial LPS quantification kit also revealed LPS in galectin preparations. Chromatography was effective in removing LPS, suggesting that such a technique needs to be applied to prevent assigning cellular responses to galectins rather than LPS.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 379, Issue 1, 30 January 2009, Pages 155–159
نویسندگان
, , , , , , , , ,