کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1934676 1050647 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A simple assay for hyaluronidase activity using fluorescence polarization
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
A simple assay for hyaluronidase activity using fluorescence polarization
چکیده انگلیسی

Hyaluronan is a large glycosaminoglycan and is a major constituent of the extracellular matrix, interacting with cell surface receptors such as CD44. We previously reported that fragmented hyaluronan, with the size frequently detected in cancer patients, induces CD44 cleavage and concomitantly enhances tumor cell migration. Although hyaluronan degradation to smaller fragments has been revealed to be a key reaction in regulating cancer progression, simple methods for continuously detecting hyaluronidase activity have not been established. Here, we show that fluorescently-labeled hyaluronan serves as a substrate for continuous assay of hyaluronidase activity. A very simple assay was established to measure degradation of hyaluronan based on fluorescence polarization. The developed assay method would provide a way for continuous measurement of cellular hyaluronidase activity and also for measurement of binding of hyaluronan to its receptors, and thus should be useful for investigation of the function of hyaluronan in cancer progression.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 376, Issue 3, 21 November 2008, Pages 620–624
نویسندگان
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