کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1935048 | 1050657 | 2009 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Regulation of matrix metalloproteinase-9 expression between gingival fibroblast cells from old and young rats Regulation of matrix metalloproteinase-9 expression between gingival fibroblast cells from old and young rats](/preview/png/1935048.png)
Gingival fibroblast cells (rGF) from aged rats have an age-related decline in proliferative capacity compared with young rats. We investigated G1 phase cell cycle regulation and MMP-9 expression in both young and aged rGF. G1 cell cycle protein levels and activity were significantly reduced in response to interleukin-1β (IL-1β) stimulation with increasing in vitro age. Tumor necrosis factor-α (TNF-α)-induced matrix metalloproteinase-9 (MMP-9) expression was also decreased in aged rGF in comparison with young rGF. Mutational analysis and gel shift assays demonstrated that the lower MMP-9 expression in aged rGF is associated with lower activities of transcription factors NF-κB and AP-1. These results suggest that cell cycle dysregulation and down-regulation of MMP-9 expression in rGF may play a role in gingival remodeling during in vitro aging.
Journal: Biochemical and Biophysical Research Communications - Volume 378, Issue 2, 9 January 2009, Pages 152–156