کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1935556 | 1050669 | 2008 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Prion early kinetics revisited using a streptomycin-based PrPres extraction method
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
The use of streptomycin in the PrPsc detection procedures represents a new and attractive way to detect more PrPsc, the best marker for the transmissible spongiform encephalopathies (TSEs). Actually, the streptomycin PrPsc aggregating property reported recently was established as beneficial in PrPsc detection using immunohistochemistry in diagnostic as well as in experimental conditions. The present study reports in details how to use advantageously this original streptomycin property in PrPres biochemical extraction and detection. Using TSE diagnostic brain material, specificity and increased sensitivity using streptomycin-treated samples were substantiated. Then an early sequential brain and spleen sampling (from 7 to 49 days post-inoculation) from C57Bl/6 mice inoculated intra-cerebrally or intra-peritoneally with C506M3 scrapie strain was analysed using streptomycin versus ultracentrifugation PrPres extraction. Whatever the inoculation route, streptomycin allowed earlier PrPres detection in spleen (7 d.p.i.), then in brain suggesting a stronger affinity of the infectious agent for the lymphoid compartment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 372, Issue 3, 1 August 2008, Pages 429-433
Journal: Biochemical and Biophysical Research Communications - Volume 372, Issue 3, 1 August 2008, Pages 429-433
نویسندگان
Edwige Leclere, Aly Moussa, Latifa Chouaf-Lakhdar, Anthony W. Coleman, Jean-Marie Seigneurin, Hervé Perron, Anna Bencsik,