کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1935861 | 1050676 | 2008 | 5 صفحه PDF | دانلود رایگان |

RNA interference is a recent, gene silencing technique that could be extremely valuable in studying gene function, treating diseases, and developing novel animal models for human diseases. Here, we investigated the feasibility of applying shRNA-mediated RNA interference in fetal fibroblasts for silencing of the myostatin gene and investigate adverse effects of RNAi. We report that up to 97% silencing of myostatin mRNA was achieved using shRNA constructs in transiently and stably transfected fetal fibroblasts (p < 0.05). At the same time we also demonstrate that high level of shRNA expression resulted in 10- to 1000-fold induction of interferon responsive genes (OAS1, IFN-β) (p < 0.05). In addition we also report novel adverse effect of shRNA expression in stably transfected cells—interference with microRNA processing/transport which led to 500-fold increase in the level of miR21 precursors (p < 0.05). Reduction of these side effects will be essential to obtain long term stable RNAi silencing.
Journal: Biochemical and Biophysical Research Communications - Volume 370, Issue 1, 23 May 2008, Pages 113–117