کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1936003 | 1050680 | 2008 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Involvement of aldolase A in X-ray resistance of human HeLa and UVr-1 cells Involvement of aldolase A in X-ray resistance of human HeLa and UVr-1 cells](/preview/png/1936003.png)
To find novel proteins involved in radio-resistance of human cells, we searched for nuclear proteins, whose expression levels alter after X-ray irradiation in HeLa cells, using agarose fluorescent two-dimensional differential gel electrophoresis following mass spectrometry. We identified 6 proteins, whose levels were increased in nuclei 24 h after irradiation at 5 Gy, including aldolase A. Nuclear aldolase A levels increased twofold after the irradiation, however, total aldolase A levels did not change. When the expression of aldolase A was suppressed by its specific siRNA, sensitization of the suppressed cells to X-ray-induced cell death was observed. In addition, UVr-1 cells with higher aldolase A expression exhibited lower sensitivity to X-ray-induced cell death than the parental RSa cells with lower aldolase A expression. These results suggest that aldolase A may play a role in the radio-response of human cells, probably in nuclei, in addition to its glycolytic role in the cytosol.
Journal: Biochemical and Biophysical Research Communications - Volume 369, Issue 3, 9 May 2008, Pages 948–952