کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1936243 | 1050687 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of the domains required for the localization of Prp19p to lipid droplets or the nucleus
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Prp19p is a protein found in the nucleus, cytosol or lipid droplets depending on the cell type. Prp19p participates in pre-mRNA splicing, in neuronal/astroglial cell fate decisions or in adipocyte lipid droplet biogenesis. In this study, the motifs of Prp19p that are necessary for its localization to lipid droplets or the nucleus in 3T3-L1 adipocytes are investigated using a series of truncated mutants of Prp19p that were fused to EGFP and transiently introduced into differentiated 3T3-L1 adipocytes. Immunofluorescence microscopy revealed that a domain of amino acids 167–250 is necessary for the recruitment of Prp19p to lipid droplets and that a domain of amino acids 1–166 is necessary for the recruitment of Prp19p to a nucleus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 364, Issue 4, 28 December 2007, Pages 844–849
Journal: Biochemical and Biophysical Research Communications - Volume 364, Issue 4, 28 December 2007, Pages 844–849
نویسندگان
Si Young Cho, Pil Joon Park, Jeong Ho Lee, Jung Joo Kim, Tae Ryong Lee,