کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1936819 | 1050703 | 2007 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Twin ribozyme mediated removal of nucleotides from an internal RNA site Twin ribozyme mediated removal of nucleotides from an internal RNA site](/preview/png/1936819.png)
Over the past two decades, the structure and mechanism of catalytic RNA have been extensively studied; now ribozymes are understood well enough to turn them into useful tools. After we have demonstrated the twin ribozyme mediated insertion of additional nucleotides into a predefined position of a suitable substrate RNA, we here show that a similar type of twin ribozyme is also capable of mediating the opposite reaction: the site-specific removal of nucleotides. In particular, we have designed a twin ribozyme that supports the deletion of four uridine residues from a given RNA substrate. This reaction is a kind of RNA recombination that in the specific context of gene therapy mimics, at the level of RNA, the correction of insertion mutations. As a result of the twin ribozyme driven reaction, 17% of substrate are converted into the four nucleotides shorter product RNA.
Journal: Biochemical and Biophysical Research Communications - Volume 363, Issue 1, 9 November 2007, Pages 24–29