کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1936861 | 1050704 | 2008 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Production and characterization of a monoclonal antibody against GPR40 (FFAR1; free fatty acid receptor 1)
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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![عکس صفحه اول مقاله: Production and characterization of a monoclonal antibody against GPR40 (FFAR1; free fatty acid receptor 1) Production and characterization of a monoclonal antibody against GPR40 (FFAR1; free fatty acid receptor 1)](/preview/png/1936861.png)
چکیده انگلیسی
GPR40 is G protein-coupled receptor whose endogenous ligands have recently been identified as free fatty acids (FFAs), and it has been implicated to play an important role in FFA-mediated enhancement of glucose-stimulated insulin release. We have developed a monoclonal antibody against the extracellular domain of GPR40. Specificity of the antibody was demonstrated by immunoprecipitation and cell surface staining using GPR40-transfected cells. GPR40 immunoreactivity was highly abundant in mouse pancreatic β-cells and splenocytes, THP-1 cells, and human peripheral blood mononuclear cells. The anti-GPR40 monoclonal antibody should prove valuable for further studying the function of this nutrient sensing receptor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 365, Issue 1, 4 January 2008, Pages 22–28
Journal: Biochemical and Biophysical Research Communications - Volume 365, Issue 1, 4 January 2008, Pages 22–28
نویسندگان
Akira Hirasawa, Chisato Itsubo, Keiko Sadakane, Takafumi Hara, Shingo Shinagawa, Hanako Koga, Hiroshi Nose, Taka-aki Koshimizu, Gozoh Tsujimoto,