کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1936903 1050705 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ile178 of HIV-1 reverse transcriptase is critical for inhibiting the viral integrase
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Ile178 of HIV-1 reverse transcriptase is critical for inhibiting the viral integrase
چکیده انگلیسی

HIV-1 reverse transcriptase (RT) was shown to inhibit in vitro the viral integrase (IN). We have reported previously that an RT-derived 20-residue peptide binds IN and inhibits its enzymatic activities. In this peptide, Leu168, Phe171, Gln174, and Ile178 were predicted to be involved in IN inhibition. In the presented study, these residues were mutagenized and the resulting peptides were tested for binding and inhibiting IN activities. Ile178 was found to be the major contributor to IN inhibition, probably by interacting with IN residue Gly149. As Gly149 is a key IN residue, this inhibition probably results from a steric hindrance of the IN active site.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 364, Issue 1, 7 December 2007, Pages 48–52
نویسندگان
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