کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1937032 1050707 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of ghrelin on human endothelial cells apoptosis induced by high glucose
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Effect of ghrelin on human endothelial cells apoptosis induced by high glucose
چکیده انگلیسی

Endothelial dysfunction is thought to be a major cause of vascular complications in diabetes. Our research shows that ghrelin attenuates high glucose-induced apoptosis in cultured human umbilical vein endothelial cells (ECV-304). Exposure to glucose (33.3 mM) for 72 h caused a significant increase in apoptosis, as evaluated by TUNEL and flow cytometry, but pretreatment of ghrelin (10−7 M) eliminated high glucose-induced apoptosis in ECV-304. Ghrelin also prevented the induction of caspase-3 activation, in cells incubated with glucose (33.3 mM). Exposure of cells to ghrelin (10−7 M) caused rapid activation of Akt. PI3K inhibitor, LY294002 attenuated ghrelin’s inhibitory effect on caspase-3 activity. Ghrelin protected endothelial cells from high glucose by inhibiting reactive oxygen species (ROS) generation. Results of our study indicate that ghrelin inhibits both high glucose-induced apoptosis via PI3K/Akt pathway and ROS production in ECV-304. This peptide may have potential in preventing diabetic complications, especially in obese patients.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 362, Issue 3, 26 October 2007, Pages 677–681
نویسندگان
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