کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1937049 1050707 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Targeting the expression of functional murine CMP-sialic acid transporter to the E. coli inner membrane
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Targeting the expression of functional murine CMP-sialic acid transporter to the E. coli inner membrane
چکیده انگلیسی

The heterologous expression of functional mammalian integral membrane proteins still represents a significant hurdle towards their crystallization and structure elucidation. We have therefore explored the use of the OmpA signal sequence to deliberately target the expression of the murine CMP-sialic acid transporter, a Golgi-resident protein with 10 putative transmembrane domains, to the Escherichia coli inner membrane. Here, we show that the expression of an OmpA signal sequence-FLAG-CMP-sialic acid transporter fusion protein in E. coli results in the targeting and insertion of recombinant protein within the inner membrane. Significantly, functionality was confirmed by the ability of spheroplasted E. coli and mixed phosphatidylcholine–E. coli inner membrane proteoliposomes incorporating recombinant CMP-sialic acid transporter to accumulate CMP-sialic acid in vitro.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 362, Issue 3, 26 October 2007, Pages 779–784
نویسندگان
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