کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1937171 1050710 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
SanM catalyzes the formation of 4-pyridyl-2-oxo-4-hydroxyisovalerate in nikkomycin biosynthesis by interacting with SanN
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
SanM catalyzes the formation of 4-pyridyl-2-oxo-4-hydroxyisovalerate in nikkomycin biosynthesis by interacting with SanN
چکیده انگلیسی

Nikkomycins are peptidyl nucleoside antibiotics with potent activities against phytopathogenic and human pathogenic fungi. The sanM and sanN genes are required for the nikkomycin biosynthesis of Streptomyces ansochromogenes. In the present study, interaction between SanM and SanN was identified by yeast two-hybrid and co-immunoprecipitation assays. Moreover, SanM and SanN were heterologously expressed and purified. Further biochemical assay demonstrated that the SanM–SanN interaction is essential for SanM aldolase activity but not for SanN dehydrogenase activity. SanM converts piconaldehyde and 2-oxobutyrate to 4-pyridyl-2-oxo-4-hydroxyisovalerate in nikkomycin biosynthesis by interacting with SanN. Steady state kinetics analysis revealed that Km and kcat/Km of SanM are 123.2 μM and 11.4 mM−1 s−1 for picolinaldehyde, while 335.6 μM and 4.0 mM−1 s−1 for 2-oxobutyrate, respectively. However, SanN as a dehydrogenase is independent of SanM.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 361, Issue 1, 14 September 2007, Pages 196–201
نویسندگان
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