کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1937325 1050714 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cardiac-specific activation of Cre expression at late fetal development
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Cardiac-specific activation of Cre expression at late fetal development
چکیده انگلیسی

In a first step towards dissecting molecular mechanisms that contribute to the development of cardiac diseases, we have generated transgenic mice that express a Cre–GFP fusion protein under the transcriptional control of a 4.3 kb murine cardiac Troponin I gene (cTnI) promoter. Cre–GFP expression, similar in three transgenic lines, is described in one line. In mouse embryos, transgenic for the Cre–GFP and ROSA lacZ reporter allele, first Cre-mediated recombination appeared at 16.5 dpc selectively at the heart. Like the endogenous cTnI gene, transgenic Cre expression showed a slow rise through fetal development that increased neonatally. Bitransgenic hearts, stained at 30 days of age, showed intense signals in ventricular and atrial myocytes while no recombination occurred in other tissues. The delayed onset of Cre activity in cTnI–Cre mice could provide a useful genetic tool to evaluate the function of loxP targeted cardiac genes without interference of recombination during early heart development.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 359, Issue 2, 27 July 2007, Pages 209–213
نویسندگان
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