کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1937390 1050715 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Alternative splicing of the OsBWMK1 gene generates three transcript variants showing differential subcellular localizations
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Alternative splicing of the OsBWMK1 gene generates three transcript variants showing differential subcellular localizations
چکیده انگلیسی

In eukaryotes, mitogen-activated protein kinases (MAPKs) play important roles in various developmental processes and in environmental stress responses. Here, we show that alternative splicing of the OsBWMK1, a member of the rice MAPK family, generates three transcript variants, OsBWMK1L, OsBWMK1M, and OsBWMK1S. The OsBWMK1L transcript variant was highly and constitutively expressed in all rice tissues tested and its expression was not altered by various stress conditions, whereas OsBWMK1M and OsBWMK1S were normally expressed at low levels but were induced by various stresses. A transient expression assay demonstrated that OsBWMK1L::GFP and OsBWMK1M::GFP were predominantly localized in the cytoplasm, whereas most OsBWMK1S::GFP was localized in the nucleus. Moreover, treatment with defense signaling related molecules, such as H2O2 and SA, induced translocation of OsBWMK1 isoforms from the cytoplasm to the nucleus. Thus, our results suggest that alternative splicing of OsBWMK1 generates three different transcript variants that produce proteins with different subcellular localizations.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 360, Issue 1, 17 August 2007, Pages 188–193
نویسندگان
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