کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1937667 | 1050722 | 2007 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: GABPα regulates Oct-3/4 expression in mouse embryonic stem cells GABPα regulates Oct-3/4 expression in mouse embryonic stem cells](/preview/png/1937667.png)
Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts, and transcription factors Oct-3/4, Nanog, Sox2, and STAT3, are essential for their self-renewal. In this study, we searched for molecules downstream of STAT3 in ES cells. Using DNA chip analysis, we obtained GA-repeat binding protein (GABP) α. Expression of GABPα was restricted to undifferentiated ES cells and controlled by STAT3. We found that the expression level of Oct-3/4 is reduced by knockdown of GABPα. On the other hand, GABPα-overexpressing ES cells maintained the expression level of Oct-3/4 even in the absence of LIF. Moreover, the induction of Oct-3/4 repressors Cdx-2, Coup-tf1, and GCNF was stimulated by GABPα knockdown. These data suggest that GABPα upregulates the expression of Oct-3/4 via downregulation of Oct-3/4 repressors.
Journal: Biochemical and Biophysical Research Communications - Volume 353, Issue 3, 16 February 2007, Pages 686–691