کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1937767 | 1050725 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cooperative binding of TIA-1 and U1 snRNP in K-SAM exon splicing activation
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Cooperative binding of TIA-1 and U1 snRNP in K-SAM exon splicing activation Cooperative binding of TIA-1 and U1 snRNP in K-SAM exon splicing activation](/preview/png/1937767.png)
چکیده انگلیسی
In 293 cells, splicing of the human fibroblast growth factor receptor-2 K-SAM alternative exon is inefficient, but can be made efficient by provoking TIA-1 binding to the U-rich IAS1 sequence downstream from the exon's 5â² splice site. We show here that TIA-1 domains known to interact with U1 snRNP and to recruit it to 5â² splice sites in vitro are required for TIA-1 activation of K-SAM exon splicing in vivo. We further show that tethering downstream from the K-SAM exon a fusion between the U1 snRNP component U1C and the bacteriophage MS2 coat protein provokes IAS1-dependent exon splicing, and present evidence that the fusion functions after its incorporation into U1 snRNP. Our in vivo data, taken together with previous in vitro results, show that K-SAM splicing activation involves cooperative binding of TIA-1 and U1 snRNP to the exon's 5â² splice site region.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 358, Issue 4, 13 July 2007, Pages 1065-1070
Journal: Biochemical and Biophysical Research Communications - Volume 358, Issue 4, 13 July 2007, Pages 1065-1070
نویسندگان
Marie-Claude Gesnel, Sandrine Theoleyre, Fabienne Del Gatto-Konczak, Richard Breathnach,