کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1937788 1050726 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The transport of α1A-adrenergic receptor with 33-nm step size in live cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The transport of α1A-adrenergic receptor with 33-nm step size in live cells
چکیده انگلیسی

We used the technique of single particle tracking (SPT) with high tempo-spatial resolution to efficiently explore the route and mechanism for the transport of α1A-adrenergic receptor (α1A-AR) in real time in living cells. We found that the initial transport of α1A-AR in cells depended on actin filaments with the velocity of 0.2 μm/s and exhibited discrete 33-nm steps. It was noted that the step size, the rate constant, and the velocities were in accordance with the character of single myosin in vitro, implying that while transporting each endosome myosins did not work in the “tug-of-war” mode and that they did not adopt the strategy to boost up transporting speed by working coordinately. These results provided insight into the mechanism of GPCR transport in vivo.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 353, Issue 2, 9 February 2007, Pages 231–237
نویسندگان
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