کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1937797 1050726 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
EOS1, whose deletion confers sensitivity to oxidative stress, is involved in N-glycosylation in Saccharomyces cerevisiae
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
EOS1, whose deletion confers sensitivity to oxidative stress, is involved in N-glycosylation in Saccharomyces cerevisiae
چکیده انگلیسی

The deletion strain of Saccharomyces cerevisiae YNL080c (designed as EOS1) was identified as a strain sensitive to high-sucrose stress in our previous report [A. Ando, F. Tanaka, Y. Murata, H. Takagi, J. Shima, Identification and classification of genes required for tolerance to high sucrose stress revealed by genome-wide screening of Saccharomyces cerevisiae, FEMS Yeast Res. 6 (2006) 249–267]. Δeos1 showed higher sensitivity to oxidative stress than to high-sucrose stress. Immunofluorescence microscopic and cellular fractionation analyses suggested that Eos1 localizes in the endoplasmic reticulum membrane. We found that the deletion of EOS1 enhances tunicamycin tolerance and that in Δeos1 the transcription level of KAR2, which is the ER stress-inducible gene, was much lower than that in the wild-type strain (BY4741) when exposed to tunicamycin. The inhibition of the N-glycosylation of carboxypeptidase Y and invertase activity caused by the addition of tunicamycin was depressed in Δeos1, suggesting that EOS1 may be involved in N-glycosylation of the cellular proteins.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 353, Issue 2, 9 February 2007, Pages 293–298
نویسندگان
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