کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1937870 | 1050727 | 2007 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Heat shock augments myosin phosphatase target-subunit phosphorylation
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Our previous study demonstrated that heat shock augmented vascular contraction. In the present study, we hypothesized that heat shock augments myosin phosphatase target-subunit (MYPT1) phosphorylation resulting in augmented vascular contraction. Endothelium-denuded rat aortic rings were mounted in organ baths, exposed to heat shock (42 °C for 45 min), and subjected to contraction 4 h after the heat shock followed by Western blot analysis for MLC20 (the 20 kDa light chains of myosin II) or MYPT1. The contractile responses in both control and heat shock-treated aorta were inhibited by Y27632, an inhibitor of Rho-kinase. The level of the MLC20 and MYPT1Thr855 phosphorylation in response to KCl was higher in heat shock-treated aorta than that in timed-control. The increased MYPT1Thr855 phosphorylation was inhibited by Y27632 (1.0 μM) in parallel with inhibition of MLC20 phosphorylation and vascular contraction. These results indicate that heat shock augments MYPT1 phosphorylation resulting in augmented vascular contraction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 356, Issue 3, 11 May 2007, Pages 718-722
Journal: Biochemical and Biophysical Research Communications - Volume 356, Issue 3, 11 May 2007, Pages 718-722
نویسندگان
Jee In Kim, Su Bun Jeon, Inji Baek, Young Mi Seok, Heung-Mook Shin, In Kyeom Kim,