کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1937975 | 1050729 | 2007 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The formation of hydrogen bond in the proximal heme pocket of HemAT-Bs upon ligand binding
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The formation of hydrogen bond in the proximal heme pocket of HemAT-Bs upon ligand binding The formation of hydrogen bond in the proximal heme pocket of HemAT-Bs upon ligand binding](/preview/png/1937975.png)
چکیده انگلیسی
HemAT-Bs is the heme-based O2 sensor responsible for aerotaxis control in Bacillus subtilis. In this study, we measured the time-resolved resonance Raman spectra of full-length HemAT-Bs wild-type (WT) and Y133F in the deoxy form and the photoproduct after photolysis of CO-bound form. In WT, the νFe-His band for the 10 ps photoproduct was observed at higher frequency by about 2 cmâ1 compared with that of the deoxy form. This frequency difference is relaxed in hundreds of picoseconds. This time-dependent frequency shift would reflect the conformational change of the protein matrix. On the other hand, Y133F mutant did not show such a substantial νFe-His frequency shift after photolysis. Since a hydrogen bond to the proximal His induces an up-shift of the νFe-His frequency, these results indicate that Tyr133 forms a hydrogen bond to the proximal His residue upon the ligand binding. We discuss a functional role of this hydrogen bond formation for the signal transduction in HemAT-Bs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 357, Issue 4, 15 June 2007, Pages 1053-1057
Journal: Biochemical and Biophysical Research Communications - Volume 357, Issue 4, 15 June 2007, Pages 1053-1057
نویسندگان
Hideaki Yoshimura, Shiro Yoshioka, Yasuhisa Mizutani, Shigetoshi Aono,