کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1938062 1050731 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structural analysis of the 26S proteasome by cryoelectron tomography
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Structural analysis of the 26S proteasome by cryoelectron tomography
چکیده انگلیسی

The 26S proteasome is the key enzyme of intracellular protein degradation in eukaryotic cells. It is a multisubunit complex of 2.5 MDa confining the proteolytic action to an inner compartment with tightly controlled access. Structural studies of this intriguing molecular machine have been hampered by its intrinsic instability and its dynamics. Here we have used an unconventional approach to obtain a three-dimensional structure of the holocomplex uncompromised by preparation-induced alterations and unbiased by any starting model. We have performed a tomographic reconstruction, followed by averaging over approx. 150 individual reconstructions, of Drosophila 26S proteasomes suspended in a thin layer of amorphous ice.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 353, Issue 1, 2 February 2007, Pages 115–120
نویسندگان
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