کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1938230 | 1050736 | 2007 | 6 صفحه PDF | دانلود رایگان |

β1,4-Galactosyltransferase II (β4GalT-II) is one of the enzymes transferring galactose to the terminal N-acetylglucosamine of complex-type N-glycans and its expression is significantly altered during oncogenesis with unknown functions. Here, we reported for the first time the pro-apoptotic role of β4GalT-II in tumor cells. The level of β4GalT-II mRNA expression was obviously decreased during HeLa cell apoptosis induced by cisplatin. Interestingly, the ectopic expression of β4GalT-II in HeLa cells markedly increased apoptosis and cleavage of PARP induced by cisplatin as well as the expression of pro-apoptotic protein Bax. Furthermore, deletion of Golgi localization domain abolished the apoptotic role of β4GalT-II in HeLa cells. Collectively, these results suggest that β4GalT-II increases HeLa cell apoptosis induced by cisplatin depending on its Golgi localization, which indicates that β4GalT-II might contribute to the therapeutic efficiency of cisplatin for cervix cancer.
Journal: Biochemical and Biophysical Research Communications - Volume 358, Issue 1, 22 June 2007, Pages 41–46