Constructing a novel Nanodevice powered by δ-free FoF1-ATPase

A Nanodevice was constructed by δ-free FoF1-ATPase within chromatophores and actin filaments through biotinlipid–streptavidin–biotin-(AC5)2Sulfo-OSu system. One actin filament linking with many chromatophores functions as the Nanodevice body and many δ-free FoF1-ATPase as the Nanodevice motors. Movement of the Nanodevice was observed directly by fluorescence microscopy with CCD camera after illumination. The moving speed was about 2.17–24.43 μm/s for various length Nanodevices and most of them were stopped by adding CCCP. This means that the Nanodevice was driven by PMF (proton-motive force) in the cooperating δ-free FoF1-ATPase. From bioengineering point of view, the cooperation of FoF1-ATPase is a very important research field in the future.