کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1938651 | 1050744 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Activation of MAPK in fibroblasts by Treponema denticola major outer sheath protein
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Activation of MAPK in fibroblasts by Treponema denticola major outer sheath protein Activation of MAPK in fibroblasts by Treponema denticola major outer sheath protein](/preview/png/1938651.png)
چکیده انگلیسی
The major outer sheath protein (Msp) of Treponema denticola induces Ca2+ entry and actin reorganization in cultured fibroblasts, but the pathways by which Msp mediates these responses are not yet defined. We considered that Msp may activate protein kinases as a stress response that precedes actin remodelling. Phospho-kinase screens showed that Msp induced phosphorylation of multiple kinases in pathways that respond to extracellular agonists and regulate actin assembly. 34 kinases were significantly activated, including p38 and ERK 1/2. Accordingly, the expression and phosphorylation of p38 and ERK 1/2 in whole cell lysates were measured by immunoblotting and densitometry. Both kinases responded in a dose- and time-dependent manner to Msp exposure, were inhibited by SB202190 and U1026, respectively, and were unaffected by extracellular Ca2+. These data indicate that T. denticola Msp may exert transient stress on fibroblasts through activation of MAP kinase pathways.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 356, Issue 1, 27 April 2007, Pages 213-218
Journal: Biochemical and Biophysical Research Communications - Volume 356, Issue 1, 27 April 2007, Pages 213-218
نویسندگان
Marie-Claude Jobin, Inderpreet Virdee, Christopher A. McCulloch, Richard P. Ellen,