کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1938722 1050745 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Phenotypic screening of hepatocyte nuclear factor (HNF) 4-γ receptor knockout mice
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Phenotypic screening of hepatocyte nuclear factor (HNF) 4-γ receptor knockout mice
چکیده انگلیسی

Using the mouse as a model organism in pharmaceutical research presents unique advantages as its physiology in many ways resembles the human physiology, it also has a relatively short generation time, low breeding and maintenance costs, and is available in a wide variety of inbred strains. The ability to genetically modify mouse embryonic stem cells to generate mouse models that better mimic human disease is another advantage. In the present study, a comprehensive phenotypic screening protocol is applied to elucidate the phenotype of a novel mouse knockout model of hepatocyte nuclear factor (HNF) 4-γ. HNF4-γ is expressed in the kidneys, gut, pancreas, and testis. The first level of the screen is aimed at general health, morphologic appearance, normal cage behaviour, and gross neurological functions. The second level of the screen looks at metabolic characteristics and lung function. The third level of the screen investigates behaviour more in-depth and the fourth level consists of a thorough pathological characterisation, blood chemistry, haematology, and bone marrow analysis. When compared with littermate wild-type mice (HNF4-γ+/+), the HNF4-γ knockout (HNF4-γ−/−) mice had lowered energy expenditure and locomotor activity during night time that resulted in a higher body weight despite having reduced intake of food and water. HNF4-γ−/− mice were less inclined to build nest and were found to spend more time in a passive state during the forced swim test.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 349, Issue 2, 20 October 2006, Pages 825–832
نویسندگان
, , , , , , , , , , , , , ,