کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1939014 | 1050752 | 2006 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Scallop lens Ω-crystallin (ALDH1A9): A novel tetrameric aldehyde dehydrogenase Scallop lens Ω-crystallin (ALDH1A9): A novel tetrameric aldehyde dehydrogenase](/preview/png/1939014.png)
Scallop eye lens Ω-crystallin is an inactive aldehyde dehydrogenase (ALDH1A9) related to cytoplasmic ALDH1A1 and mitochondrial ALDH2 that migrates by gel filtration chromatography as a homodimer. Because mammalian ALDH1A1 and ALDH2 are homotetramers, we investigated the native molecular mass of scallop Ω-crystallin by multi-angle laser light scattering. The results indicate that the scallop Ω-crystallin is a tetrameric, not a dimeric protein. Moreover, phylogenetic tree analysis shows that scallop Ω-crystallin clusters with the mitochondrial ALDH2 and ALDH1B1 rather than the cytoplasmic ALDH1A, yet it lacks the mitochondrial N-terminal leader sequence characteristic of the mitochondrial ALDHs. The mitochondrial grouping, enzymatic inactivity, and anomalous gel filtration behavior make scallop cytoplasmic Ω-crystallin an interesting protein for structural studies of evolutionary adaptations to become an enzyme-crystallin.
Journal: Biochemical and Biophysical Research Communications - Volume 348, Issue 4, 6 October 2006, Pages 1302–1309