کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1939028 | 1050752 | 2006 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A lentiviral vector with expression controlled by E2F-1: A potential tool for the study and treatment of proliferative diseases A lentiviral vector with expression controlled by E2F-1: A potential tool for the study and treatment of proliferative diseases](/preview/png/1939028.png)
We have constructed a lentiviral vector with expression limited to cells presenting active E2F-1 protein, a potential advantage for gene therapy of proliferative diseases. For the FE2FLW vector, the promoter region of the human E2F-1 gene was utilized to drive expression of luciferase cDNA, included as a reporter of viral expression. Primary, immortalized, and transformed cells were transduced with the FE2FLW vector and cell cycle alterations were induced with serum starvation/replacement, contact inhibition or drug treatment, revealing cell cycle-dependent changes in reporter activity. Forced E2F-1 expression, but not E2F-2 or E2F-3, increased reporter activity, indicating a major role for this factor in controlling expression from the FE2FLW virus. We show the utility of this vector as a reporter of E2F-1 and proliferation-dependent cellular alterations upon cytotoxic/cytostatic treatment, such as the introduction of tumor suppressor genes. We propose that the FE2FLW vector may be a starting point for the development of gene therapy strategies for proliferative diseases, such as cancer or restinosis.
Journal: Biochemical and Biophysical Research Communications - Volume 348, Issue 4, 6 October 2006, Pages 1411–1418