کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1939355 1050759 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of photo-immobilization of epidermal growth factor on the cellular behaviors
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Effect of photo-immobilization of epidermal growth factor on the cellular behaviors
چکیده انگلیسی

We constructed photo-reactive epidermal growth factor (EGF) bearing p-azido phenylalanine at the C-terminal (HEGFP) by genetic engineering to investigate the possibility of immobilized EGF as a novel artificial extracellular matrix (ECM). The constructed recombinant protein was immobilized to glass surface by ultraviolet irradiation. A431 cells adhered both to HEGFP-immobilized and collagen-coated surfaces. Interaction between immobilized HEGFP and EGF receptors in the A431 cells was independent of Mg2+ although integrin-mediated cell adhesion to natural ECMs is dependent on Mg2+. Phosphorylation of EGF receptors in A431 cells was induced by immobilized HEGFP as same as soluble EGF. DNA uptake of hepatocytes decreased by immobilized HEGFP whereas it increased by soluble EGF. Liver-specific functions of hepatocytes were maintained for 3 days by immobilized HEGFP whereas they were not maintained by soluble EGF, indicating that immobilized HEGFP follows different signal transduction pathway from soluble EGF.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 345, Issue 1, 23 June 2006, Pages 255–259
نویسندگان
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