کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1939706 1050765 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chlamydial DNA polymerase I can bypass lesions in vitro
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Chlamydial DNA polymerase I can bypass lesions in vitro
چکیده انگلیسی

We found that DNA polymerase I from Chlamydiophila pneumoniae AR39 (CpDNApolI) presents DNA-dependent DNA polymerase activity, but has no detectable 3′ exonuclease activity. CpDNApolI-dependent DNA synthesis was performed using DNA templates carrying different lesions. DNAs containing 2′-deoxyuridine (dU), 2′-deoxyinosine (dI) or 2′-deoxy-8-oxo-guanosine (8-oxo-dG) served as templates as effectively as unmodified DNAs for CpDNApolI. Furthermore, the CpDNApolI could bypass natural apurinic/apyrimidinic sites (AP sites), deoxyribose (dR), and synthetic AP site tetrahydrofuran (THF). CpDNApolI could incorporate any dNMPs opposite both of dR and THF with the preference to dAMP-residue. CpDNApolI preferentially extended primer with 3′-dAMP opposite dR during DNA synthesis, however all four primers with various 3′-end nucleosides (dA, dT, dC, and dG) opposite THF could be extended by CpDNApolI. Efficiently bypassing of AP sites by CpDNApolI was hypothetically attributed to lack of 3′ exonuclease activity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 345, Issue 3, 7 July 2006, Pages 1083–1091
نویسندگان
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