کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1939934 | 1050770 | 2006 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: In vitro panning of a targeting peptide to hepatocarcinoma from a phage display peptide library In vitro panning of a targeting peptide to hepatocarcinoma from a phage display peptide library](/preview/png/1939934.png)
Phage display technology has been used as a powerful tool in the discovery of ligands specific to receptor(s) on the surface of a cancer cell and could also impact clinical issues including functional diagnosis and cell-specific drug delivery. After three rounds of in vitro panning and two rounds of reverse absorption, a group of phages capable of addressing BEL-7402 enormously were obtained for further analysis. Through a cell-based ELISA, immunofluorescence, FACS, and in vivo binding study, WP05 (sequence TACHQHVRMVRP) was demonstrated to be the most effective peptide in targeting four kinds of liver cancer cell lines (BEL-7402, BEL-7404, SMMC-7721, and HepG2), but not the normal liver cell line HL-7702. In conclusion, the peptide WP05 which was screened by in vitro phage display technology was proved to be a targeting peptide to several common hepatocellular carcinoma cell lines.
Journal: Biochemical and Biophysical Research Communications - Volume 342, Issue 3, 14 April 2006, Pages 956–962