کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1939978 | 1050771 | 2006 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The identification and characterisation of a functional interaction between arginyl-tRNA-protein transferase and topoisomerase II The identification and characterisation of a functional interaction between arginyl-tRNA-protein transferase and topoisomerase II](/preview/png/1939978.png)
Topoisomerase II is required for the viability of all eukaryotic cells. It plays important roles in DNA replication, recombination, chromosome segregation, and the maintenance of the nuclear scaffold. Proteins that interact with and regulate this essential enzyme are of great interest. To investigate the role of proteins interacting with the N-terminal domain of the Saccharomyces cerevisiae topoisomerase II, we used a yeast two-hybrid protein interaction screen. We identified an interaction between arginyl-tRNA-protein transferase (Ate1) and the N-terminal domain of the S. cerevisiae topoisomerase II, including the potential site of interaction. Ate1 is a component of the N-end rule protein degradation pathway which targets proteins for degradation. We also propose a previously unidentified role for Ate1 in modulating the level of topoisomerase II through the cell cycle.
Journal: Biochemical and Biophysical Research Communications - Volume 342, Issue 2, 7 April 2006, Pages 596–604