کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1940001 | 1050772 | 2006 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Differential sensitivity of human platelet P2X1 and P2Y1 receptors to disruption of lipid rafts Differential sensitivity of human platelet P2X1 and P2Y1 receptors to disruption of lipid rafts](/preview/png/1940001.png)
ATP-stimulated P2X1 and ADP-stimulated P2Y1 receptors play important roles in platelet activation. An increase in intracellular Ca2+ represents a key signalling event coupled to both of these receptors, mediated via direct gating of Ca2+-permeable channels in the case of P2X1 and phospholipase-C-dependent Ca2+ mobilisation for P2Y1. We show that disruption of cholesterol-rich membrane lipid rafts reduces P2X1 receptor-mediated calcium increases by ∼80%, while P2Y1 receptor-dependent Ca2+ release is unaffected. In contrast to artery, vas deferens, bladder smooth muscle, and recombinant expression in cell lines, where P2X1 receptors show almost exclusive association with lipid rafts, only ∼20% of platelet P2X1 receptors are co-expressed with the lipid raft marker flotillin-2. We conclude that lipid rafts play a significant role in the regulation of P2X1 but not P2Y1 receptors in human platelets and that a reserve of non-functional P2X1 receptors may exist.
Journal: Biochemical and Biophysical Research Communications - Volume 343, Issue 2, 5 May 2006, Pages 415–419