کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1940193 | 1050776 | 2006 | 7 صفحه PDF | دانلود رایگان |
HuC, a vertebrate ortholog of Drosophila elav gene, encodes an RNA binding protein and is involved in early neurogenesis. Zebrafish huC is expressed in distinct neurons, including Rohon-Beard (RB) sensory neurons, interneurons and motoneurons, during primary neurogenesis, and in all neurons later during secondary neurogenesis. In this study, we identify two GC-rich box elements, proximal GC (p-GC) box from −172 to −149 and distal GC (d-GC) box from −218 to −208, in zebrafish huC promoter. Using transgenic approach, we demonstrate that deletion of the p-GC box from the promoter results in loss of expression of the reporter GFP in neurons while deletion of the d-GC box leads to GFP expression only in dorsal RB sensory neurons. These results suggest that the p-GC box alone confers transcriptional activity of huC promoter in primary RB neurons and the d-GC is required for huC transcription in the full spectrum of spinal cord neurons. Further studies are needed to identify specific Sp1-like transcription factors that bind to these GC boxes and activate huC transcription.
Journal: Biochemical and Biophysical Research Communications - Volume 342, Issue 1, 31 March 2006, Pages 214–220