کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1941078 | 1050797 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Universal initiator nucleotides for the enzymatic synthesis of 5′-amino- and 5′-thiol-modified RNA
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Universal initiator nucleotides for the enzymatic synthesis of 5′-amino- and 5′-thiol-modified RNA Universal initiator nucleotides for the enzymatic synthesis of 5′-amino- and 5′-thiol-modified RNA](/preview/png/1941078.png)
چکیده انگلیسی
We report the chemical synthesis of 5′-amino- and 5′-thiol-hexaethylene glycol guanosine nucleotides and their enzymatic incorporation into RNA, followed by chemical modifications at their nucleophilic ends. By using two similar routes, the conjugates of guanosine-5′-monophosphate and hexaethylene glycol with attached reactive groups (SH or NH2) were synthesized using phosphoramidite chemistry, and characterized by MALDI TOF mass spectrometry. These initiator molecules were efficiently incorporated into RNA at the 5′-end by run-off transcription using T7 RNA polymerase. The potential of these RNA conjugates for a broad reaction range with electrophiles is shown here, thereby enabling their use for diverse biochemical applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 344, Issue 3, 9 June 2006, Pages 887–892
Journal: Biochemical and Biophysical Research Communications - Volume 344, Issue 3, 9 June 2006, Pages 887–892
نویسندگان
Jörg C. Schlatterer, Andres Jäschke,