کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1941171 1050800 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Monochloramine potently inhibits arachidonic acid metabolism in rat platelets
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Monochloramine potently inhibits arachidonic acid metabolism in rat platelets
چکیده انگلیسی

In the present study, the effects of hypochlorous acid (HOCl), monochloramine (NH2Cl), glutamine-chloramine (Glu-Cl) and taurine-chloramine (Tau-Cl) on the formation of 12-lipoxygenase (LOX) metabolite, 12-HETE, and cyclooxygenase (COX) metabolites, TXB2, and 12-HHT, from exogenous arachidonic acid (AA) in rat platelets were examined. Rat platelets (4 × 108/ml) were preincubated with drugs for 5 min at 37 °C prior to the incubation with AA (40 μM) for 2 min at 37 °C. HOCl (50–250 μM) showed an inhibition on the formation of LOX metabolite (12-HETE, 5–67% inhibition) and COX metabolites (TXB2, 33–73% inhibition; 12-HHT, 27–74% inhibition). Although Tau-Cl and Glu-Cl up to 100 μM were without effect on the formation of 12-HETE, TXB2 and 12-HTT, NH2Cl showed a strong inhibition on the formation of all three metabolites (10–100 μM NH2Cl, 12-HETE, 21–92% inhibition; TXB2, 58–94% inhibition; 12-HHT, 36–92% inhibition). Methionine reversed a reduction of formation of LOX and COX metabolites induced by NH2Cl, and taurine restoring that induced by both NH2Cl and HOCl. These results suggest that NH2Cl is a more potent inhibitor of COX and LOX pathways in platelets than HOCl, and taurine and methionine can be modulators of NH2Cl-induced alterations in the COX and LOX pathways in vivo.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 344, Issue 1, 26 May 2006, Pages 140–145
نویسندگان
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