کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1941224 1050805 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Direct inhibition of a PKA inhibitor, H-89 on KV channels in rabbit coronary arterial smooth muscle cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Direct inhibition of a PKA inhibitor, H-89 on KV channels in rabbit coronary arterial smooth muscle cells
چکیده انگلیسی

We examined the effects of the protein kinase A (PKA) inhibitor H-89 on voltage-dependent K+ (KV) currents in freshly isolated rabbit coronary arterial smooth muscle cells, using a whole-cell patch clamp technique. H-89 inhibited the KV current in a concentration-dependent manner, with a Kd value of 1.02 μM. However, the PKA inhibitors KT 5720 and Rp-8-CPT-cAMPS did not significantly alter the KV current or the inhibitory effects of H-89 on the KV current. Moreover, H-85, a structurally similar but inactive analog of H-89, showed similar inhibitory effects on the KV channel. H-89 had no effect on the voltage-dependency of activation or inactivation, or on recovery kinetics. These results suggest that in rabbit coronary arterial smooth muscle cells, H-89 inhibits the KV current directly by blocking the pore cavity, an effect independent of PKA inhibition.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 341, Issue 4, 24 March 2006, Pages 931–937
نویسندگان
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