کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1942235 | 1052597 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Acetate in mixotrophic growth medium affects photosystem II in Chlamydomonas reinhardtii and protects against photoinhibition
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کلمات کلیدی
HSM2,6-dichloro-1,4-benzoquinoneprimary electron donor in PSIIP680PheoDCMUDCBQChlamydomonas reinhardtiiChl2,2,6,6-Tetramethyl-4-piperidone - 2،2،6،6-تترامتیل-4-پیپریدون3-(3,4-Dichlorophenyl)-1,1-dimethylurea - 3- (3،4-Dichlorophenyl) -1،1-dimethylureaAcetate - استاتSinglet oxygen - اکسیژن مجزاLHC - برخورد دهنده بزرگ هادرون Chlorophyll - سبزینه یا کلروفیلPhotosystem - سیستم عکسTAP - ضربه زدنelectron paramagnetic resonance spectroscopy - طیف سنجی رزونانس پارامغناطیسی الکترونPhotoinhibition - عکسبرداریLight harvesting complex - مجتمع برداشت نورmidpoint redox potential - پودر reddo midpointThermoluminescence - گرماتابی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Chlamydomonas reinhardtii is a photoautotrophic green alga, which can be grown mixotrophically in acetate-supplemented media (Tris-acetate-phosphate). We show that acetate has a direct effect on photosystem II (PSII). As a consequence, Tris-acetate-phosphate-grown mixotrophic C. reinhardtii cultures are less susceptible to photoinhibition than photoautotrophic cultures when subjected to high light. Spin-trapping electron paramagnetic resonance spectroscopy showed that thylakoids from mixotrophic C. reinhardtii produced less 1O2 than those from photoautotrophic cultures. The same was observed in vivo by measuring DanePy oxalate fluorescence quenching. Photoinhibition can be induced by the production of 1O2 originating from charge recombination events in photosystem II, which are governed by the midpoint potentials (Em) of the quinone electron acceptors. Thermoluminescence indicated that the Em of the primary quinone acceptor (QA/QAâ) of mixotrophic cells was stabilised while the Em of the secondary quinone acceptor (QB/QBâ) was destabilised, therefore favouring direct non-radiative charge recombination events that do not lead to 1O2 production. Acetate treatment of photosystem II-enriched membrane fragments from spinach led to the same thermoluminescence shifts as observed in C. reinhardtii, showing that acetate exhibits a direct effect on photosystem II independent from the metabolic state of a cell. A change in the environment of the non-heme iron of acetate-treated photosystem II particles was detected by low temperature electron paramagnetic resonance spectroscopy. We hypothesise that acetate replaces the bicarbonate associated to the non-heme iron and changes the environment of QA and QB affecting photosystem II charge recombination events and photoinhibition.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1827, Issue 10, October 2013, Pages 1183-1190
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1827, Issue 10, October 2013, Pages 1183-1190
نویسندگان
Thomas Roach, Arezki Sedoud, Anja Krieger-Liszkay,