The Na+-translocating F1FO-ATPase from the halophilic, alkalithermophile Natranaerobius thermophilus

► Characterize F-ATPase of halophilic alkalithermophilic Natranaerobius thermophilus. ► F-type ATPase is Na+-coupled, hydrolysis stimulated by Na+ ions. ► Maximal ATP hydrolysis activity at high temperature (47 °C) and alkaline pH (9.3). ► ATPase functions mainly in hydrolysis direction, very low rates of ATP synthesis. ► ATP hydrolysis activity regulated by untruncated epsilon subunit.