کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1942803 1052630 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dual localization of plant glutamate receptor AtGLR3.4 to plastids and plasmamembrane
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Dual localization of plant glutamate receptor AtGLR3.4 to plastids and plasmamembrane
چکیده انگلیسی

Bioinformatic approaches have allowed the identification in Arabidopsis thaliana of twenty genes encoding for homologues of animal ionotropic glutamate receptors (iGLRs). Some of these putative receptor proteins, grouped into three subfamilies, have been located to the plasmamembrane, but their possible location in organelles has not been investigated so far. In the present work we provide multiple evidence for the plastid localization of a glutamate receptor, AtGLR3.4, in Arabidopsis and tobacco. Biochemical analysis was performed using an antibody shown to specifically recognize both the native protein in Arabidopsis and the recombinant AtGLR3.4 fused to YFP expressed in tobacco. Western blots indicate the presence of AtGLR3.4 in both the plasmamembrane and in chloroplasts. In agreement, in transformed Arabidopsis cultured cells as well as in agroinfiltrated tobacco leaves, AtGLR3.4::YFP is detected both at the plasmamembrane and at the plastid level by confocal microscopy. The photosynthetic phenotype of mutant plants lacking AtGLR3.4 was also investigated. These results identify for the first time a dual localization of a glutamate receptor, revealing its presence in plastids and chloroplasts and opening the way to functional studies.

Research Highlights
► Intracellular location of a putative ionotropic glutamate receptor
► AtGLR3.4 glutamate receptor is located to both chloroplasts and plasmamembrane
► AtGLR3.4 is expressed in green tissues but not in the root
► AtGLR3.4 is located to chloroplast envelope but not to thylakoids
► Mutants lacking AtGLR3.4 shows a slight decrease in photosynthetic efficiency

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1807, Issue 3, March 2011, Pages 359–367
نویسندگان
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