کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1944744 | 1053239 | 2010 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The stimulatory effect of angiotensin II on Na+-ATPase activity involves sequential activation of phospholipases and sustained PKC activity The stimulatory effect of angiotensin II on Na+-ATPase activity involves sequential activation of phospholipases and sustained PKC activity](/preview/png/1944744.png)
Angiotensin II (Ang II) stimulates the proximal tubule Na+-ATPase through the AT1 receptor/phosphoinositide phospholipase Cβ (PI-PLCβ)/protein kinase C (PKC) pathway. However, this pathway alone does not explain the sustained effect of Ang II on Na+-ATPase activity for 30 min. The aim of the present work was to elucidate the molecular mechanisms involved in the sustained effect of Ang II on Na+-ATPase activity. Ang II induced fast and correlated activation of Na+-ATPase and PKC activities with the maximal effect (115%) observed at 1 min and sustained for 30 min, indicating a pivotal role of PKC in the modulation of Na+-ATPase by Ang II. We observed that the sustained activation of PKC by Ang II depended on the sequential activation of phospholipase D and Ca2+-insensitive phospholipase A2, forming phosphatidic acid and lysophosphatidic acid, respectively. The results indicate that PKC could be the final target and an integrator molecule of different signaling pathways triggered by Ang II, which could explain the sustained activation of Na+-ATPase by Ang II.
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1798, Issue 3, March 2010, Pages 354–359