کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1945286 | 1053259 | 2008 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: NMR investigations of interactions between anesthetics and lipid bilayers NMR investigations of interactions between anesthetics and lipid bilayers](/preview/png/1945286.png)
Interactions between anesthetics (lidocaine and short chain alcohols) and lipid membranes formed by dimyristoylphosphatidylcholine (DMPC) were studied using NMR spectroscopy. The orientational order of lidocaine was investigated using deuterium NMR on a selectively labelled compound whereas segmental ordering in the lipids was probed by two-dimensional 1H–13C separated local field experiments under magic-angle spinning conditions. In addition, trajectories generated in molecular dynamics (MD) computer simulations were used for interpretation of the experimental results. Separate simulations were carried out with charged and uncharged lidocaine molecules. Reasonable agreement between experimental dipolar interactions and the calculated counterparts was observed. Our results clearly show that charged lidocaine affects significantly the lipid headgroup. In particular the ordering of the lipids is increased accompanied by drastic changes in the orientation of the P–N vector in the choline group.
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1778, Issue 11, November 2008, Pages 2604–2611