کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1945932 1053280 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The membrane-induced structure of melittin is correlated with the fluidity of the lipids
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The membrane-induced structure of melittin is correlated with the fluidity of the lipids
چکیده انگلیسی

The effect of the bee toxin melittin on DMPC dynamics in fast-tumbling bicelles has been investigated. The 13C R1 and 13C–1H NOE relaxation parameters for DMPC were used to monitor the effect of melittin and cholesterol on lipid dynamics. It was found that melittin has the largest effect on the DMPC mobility in DMPC/DHPC bicelles, while less effect was observed in cholesterol-doped bicelles, or in bicelles made with CHAPS, indicating that the rigidity of the membrane affects the melittin–membrane interaction. CD spectra were analysed in terms of cooperativity of the α-helix to random coil transition in melittin, and these results also indicated similar differences between the bicelles. The study shows that bicelles can be used to investigate lipid dynamics by spin relaxation, and in particular of peptide-induced changes in membrane fluidity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1768, Issue 1, January 2007, Pages 115–121
نویسندگان
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