Interscapular brown adipose tissue metabolic reprogramming during cold acclimation: Interplay of HIF-1α and AMPKα

► Protein level of metabolic enzymes decreased during acute cold-exposure. ► Protein level of respiratory chain components decreased during 7-day cold-exposure. ► Levels of all abovementioned enzymes raised in the late phase of cold acclimation. ► Orchestrated protein expressions of AMPKα and HIF-1α during 45 days of cold exposure. ► NO stimulates expression of metabolic and enzymes of oxidative phosphorylation.