کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1947855 1054657 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cell-directed-assembly: Directing the formation of nano/bio interfaces and architectures with living cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Cell-directed-assembly: Directing the formation of nano/bio interfaces and architectures with living cells
چکیده انگلیسی

BackgroundThe desire to immobilize, encapsulate, or entrap viable cells for use in a variety of applications has been explored for decades. Traditionally, the approach is to immobilize cells to utilize a specific functionality of the cell in the system.Scope of reviewThis review describes our recent discovery that living cells can organize extended nanostructures and nano-objects to create a highly biocompatible nano//bio interface [1].Major conclusionsWe find that short chain phospholipids direct the formation of thin film silica mesophases during evaporation-induced self-assembly (EISA) [2], and that the introduction of cells alter the self-assembly pathway. Cells organize an ordered lipid-membrane that forms a coherent interface with the silica mesophase that is unique in that it withstands drying—yet it maintains accessibility to molecules introduced into the 3D silica host. Cell viability is preserved in the absence of buffer, making these constructs useful as standalone cell-based sensors. In response to hyperosmotic stress, the cells release water, creating a pH gradient which is maintained within the nanostructured host and serves to localize lipids, proteins, plasmids, lipidized nanocrystals, and other components at the cellular surface. This active organization of the bio/nano interface can be accomplished during ink-jet printing or selective wetting–processes allowing patterning of cellular arrays–and even spatially-defined genetic modification.General significanceRecent advances in the understanding of nanotechnology and cell biology encourage the pursuit of more complex endeavors where the dynamic interactions of the cell and host material act symbiotically to obtain new, useful functions.This article is part of a Special Issue entitled Nanotechnologies - Emerging Applications in Biomedicine.

Research Highlights
► Cells serve as living colloids – creating localized chemical potential gradients that can direct self-assembly and self-catalyze fabrication of 3D microenvironments.
► Within cell built microenvironments, chemical and mechanical ‘signaling’ reinforced by cellular confinement induces genetic reprogramming and a spectrum of complex and potentially unknown cellular behaviors.
► Non-replicative persistence/permanent senescence developed in encapsulated cells may be an ideal state, allowing basic functionality at a very low metabolic cost – of potential interest for integrated cellular devices and platforms.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1810, Issue 3, March 2011, Pages 259–267
نویسندگان
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